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vesicular stomatitis virus glycoprotein  (Addgene inc)


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    Structured Review

    Addgene inc vesicular stomatitis virus glycoprotein
    Vesicular Stomatitis Virus Glycoprotein, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 634 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/vesicular+stomatitis+virus+glycoprotein/VSV%2EG+(Plasmid+%2314888)/pmc12802833-187-33-38
    Average 96 stars, based on 634 article reviews
    vesicular stomatitis virus glycoprotein - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: Ancestral reconstruction supports that loss of Nef-mediated T cell modulation coincided with the emergence of pathogenic lentiviruses
    Article Snippet: .. The pCMV-DR8.2 plasmid (Addgene, Waterdown, MA, USA; Cat#12263, gift from Didier Trono) encoding Gag/Pol and the pMD2.G plasmid encoding vesicular stomatitis virus glycoprotein (VSV-G; Addgene, Cat#12259, gift from Didier Trono) were also used for transfections where indicated. ..

    Article Title: Ancestral reconstruction supports that loss of Nef-mediated T cell modulation coincided with the emergence of pathogenic lentiviruses.
    Article Snippet: .. The pCMV-DR8.2 plasmid (Addgene, Waterdown, MA, USA; Cat#12263, gift from Didier Trono) encoding Gag/Pol and the pMD2.G plasmid encoding vesicular stomatitis virus glycoprotein (VSV-G; Addgene, Cat#12259, gift from Didier Trono) were also used for transfections where indicated. ..

    Article Title: Development of a monoclonal antibody to study MARCH6, an E3 ligase that regulates proteins that control lipid homeostasis
    Article Snippet: .. On day 1, cells were transfected with three plasmids: i) 5 μg puromycin-selectable lentiCRISPRv2 plasmid encoding a guide targeting either exon 15 or exon 4 of MARCH6 as described above; ii) 3 μg psPAX2 plasmid encoding HIV gag polymerase (Addgene, Cat# 12260); and iii) 2 μg pMD2.G plasmid encoding vesicular stomatitis virus glycoprotein (Addgene, Cat# 12259). .. Transfections were carried out using X-tremeGENE HP (Sigma, Cat# XTGHP-RO) according to the manufacturer’s protocol.

    Article Title: YAP/TAZ-VGLL3 governs adipocyte fate via epigenetic reprogramming of PPARγ and its target enhancers
    Article Snippet: .. For generation of recombinant viruses, HEK293T cells were transfected with the use of PEI MAX (24765-1, Polysciences) for 48 hours either with a transfer plasmid together with Gag/Pol (#14887, Addgene; RRID: Addgene_14887) and vesicular stomatitis virus glycoprotein (#14888, Addgene; RRID: Addgene_14888) plasmids for retrovirus production or with psPAX2 (#12260, Addgene; RRID: Addgene_12260) and pMD2.G (#12259, Addgene; RRID: Addgene_12259) for lentivirus production. .. The virus-containing culture supernatants were harvested for infection of C3H10T1/2 cells for 24 hours in the presence of polybrene (H9268, Sigma-Aldrich) at 6 μg/ml.

    Article Title: Matrix Rigidity Controls Endothelial Differentiation and Morphogenesis of Cardiac Precursors
    Article Snippet: The p190RhoGAP overexpression plasmid was prepared by cloning the human open reading frame(ORF)segment (OHS1770) (Open Biosystems) in a simian virus 40 (SV40) lentiviral expression vector. .. Lentiviral shRNA transduction Human embryonic kidney (HEK) cells were plated at 50% confluency and transfected with pLKO1-based shRNA expression plasmid, vesicular stomatitis virus glycoprotein (VSVG) (Addgene, 8454), and Δ8.2 dvpr (Addgene, 8455) with FuGENE 6 (Roche, 11814443001). ..

    Article Title: Development of a monoclonal antibody to study MARCH6, an E3 ligase that regulates proteins that control lipid homeostasis.
    Article Snippet: .. On day 1, cells were transfected with three plasmids: i) 5 μg puromycin-selectable lentiCRISPRv2 plasmid encoding a guide targeting either exon 15 or exon 4 of MARCH6 as described above; ii) 3 μg psPAX2 plasmid encoding HIV gag polymerase (Addgene, Cat# 12260); and iii) 2 μg pMD2.G plasmid encoding vesicular stomatitis virus glycoprotein (Addgene, Cat# 12259). .. Transfections were carried out using X-tremeGENE HP (Sigma, Cat# XTGHPRO) according to the manufacturer’s protocol.

    Virus:

    Article Title: Ancestral reconstruction supports that loss of Nef-mediated T cell modulation coincided with the emergence of pathogenic lentiviruses
    Article Snippet: .. The pCMV-DR8.2 plasmid (Addgene, Waterdown, MA, USA; Cat#12263, gift from Didier Trono) encoding Gag/Pol and the pMD2.G plasmid encoding vesicular stomatitis virus glycoprotein (VSV-G; Addgene, Cat#12259, gift from Didier Trono) were also used for transfections where indicated. ..

    Article Title: Ancestral reconstruction supports that loss of Nef-mediated T cell modulation coincided with the emergence of pathogenic lentiviruses.
    Article Snippet: .. The pCMV-DR8.2 plasmid (Addgene, Waterdown, MA, USA; Cat#12263, gift from Didier Trono) encoding Gag/Pol and the pMD2.G plasmid encoding vesicular stomatitis virus glycoprotein (VSV-G; Addgene, Cat#12259, gift from Didier Trono) were also used for transfections where indicated. ..

    Article Title: Development of a monoclonal antibody to study MARCH6, an E3 ligase that regulates proteins that control lipid homeostasis
    Article Snippet: .. On day 1, cells were transfected with three plasmids: i) 5 μg puromycin-selectable lentiCRISPRv2 plasmid encoding a guide targeting either exon 15 or exon 4 of MARCH6 as described above; ii) 3 μg psPAX2 plasmid encoding HIV gag polymerase (Addgene, Cat# 12260); and iii) 2 μg pMD2.G plasmid encoding vesicular stomatitis virus glycoprotein (Addgene, Cat# 12259). .. Transfections were carried out using X-tremeGENE HP (Sigma, Cat# XTGHP-RO) according to the manufacturer’s protocol.

    Article Title: YAP/TAZ-VGLL3 governs adipocyte fate via epigenetic reprogramming of PPARγ and its target enhancers
    Article Snippet: .. For generation of recombinant viruses, HEK293T cells were transfected with the use of PEI MAX (24765-1, Polysciences) for 48 hours either with a transfer plasmid together with Gag/Pol (#14887, Addgene; RRID: Addgene_14887) and vesicular stomatitis virus glycoprotein (#14888, Addgene; RRID: Addgene_14888) plasmids for retrovirus production or with psPAX2 (#12260, Addgene; RRID: Addgene_12260) and pMD2.G (#12259, Addgene; RRID: Addgene_12259) for lentivirus production. .. The virus-containing culture supernatants were harvested for infection of C3H10T1/2 cells for 24 hours in the presence of polybrene (H9268, Sigma-Aldrich) at 6 μg/ml.

    Article Title: Matrix Rigidity Controls Endothelial Differentiation and Morphogenesis of Cardiac Precursors
    Article Snippet: The p190RhoGAP overexpression plasmid was prepared by cloning the human open reading frame(ORF)segment (OHS1770) (Open Biosystems) in a simian virus 40 (SV40) lentiviral expression vector. .. Lentiviral shRNA transduction Human embryonic kidney (HEK) cells were plated at 50% confluency and transfected with pLKO1-based shRNA expression plasmid, vesicular stomatitis virus glycoprotein (VSVG) (Addgene, 8454), and Δ8.2 dvpr (Addgene, 8455) with FuGENE 6 (Roche, 11814443001). ..

    Article Title: Development of a monoclonal antibody to study MARCH6, an E3 ligase that regulates proteins that control lipid homeostasis.
    Article Snippet: .. On day 1, cells were transfected with three plasmids: i) 5 μg puromycin-selectable lentiCRISPRv2 plasmid encoding a guide targeting either exon 15 or exon 4 of MARCH6 as described above; ii) 3 μg psPAX2 plasmid encoding HIV gag polymerase (Addgene, Cat# 12260); and iii) 2 μg pMD2.G plasmid encoding vesicular stomatitis virus glycoprotein (Addgene, Cat# 12259). .. Transfections were carried out using X-tremeGENE HP (Sigma, Cat# XTGHPRO) according to the manufacturer’s protocol.

    Article Title: Serine protease 35 regulates the fibroblast matrisome in response to hyperosmotic stress.
    Article Snippet: Plasmids with nontargeting (#VSC11657; Horizon Discovery, Waterbeach, UK) and PRSS35-targeting (#V3SH11252229744426 and #V3SH11252-230227744; Horizon Discovery) shRNAs were commercially obtained. .. Lentiviruses packaged with PAX2 (#12260; Addgene) and vesicular stomatitis virus glycoprotein (#8454; Addgene) were collected from human embryonic kidney 293T cell supernatants 2 days after transfection. .. Viral supernatant was filtered through a 0.45-μm filter, supplemented with polybrene (8 μg/ml; #sc-134220; Santa Cruz Biotechnology, Santa Cruz, CA) and used for overnight (O/N) treatment of HDFs.

    Transfection:

    Article Title: Ancestral reconstruction supports that loss of Nef-mediated T cell modulation coincided with the emergence of pathogenic lentiviruses
    Article Snippet: .. The pCMV-DR8.2 plasmid (Addgene, Waterdown, MA, USA; Cat#12263, gift from Didier Trono) encoding Gag/Pol and the pMD2.G plasmid encoding vesicular stomatitis virus glycoprotein (VSV-G; Addgene, Cat#12259, gift from Didier Trono) were also used for transfections where indicated. ..

    Article Title: Ancestral reconstruction supports that loss of Nef-mediated T cell modulation coincided with the emergence of pathogenic lentiviruses.
    Article Snippet: .. The pCMV-DR8.2 plasmid (Addgene, Waterdown, MA, USA; Cat#12263, gift from Didier Trono) encoding Gag/Pol and the pMD2.G plasmid encoding vesicular stomatitis virus glycoprotein (VSV-G; Addgene, Cat#12259, gift from Didier Trono) were also used for transfections where indicated. ..

    Article Title: Development of a monoclonal antibody to study MARCH6, an E3 ligase that regulates proteins that control lipid homeostasis
    Article Snippet: .. On day 1, cells were transfected with three plasmids: i) 5 μg puromycin-selectable lentiCRISPRv2 plasmid encoding a guide targeting either exon 15 or exon 4 of MARCH6 as described above; ii) 3 μg psPAX2 plasmid encoding HIV gag polymerase (Addgene, Cat# 12260); and iii) 2 μg pMD2.G plasmid encoding vesicular stomatitis virus glycoprotein (Addgene, Cat# 12259). .. Transfections were carried out using X-tremeGENE HP (Sigma, Cat# XTGHP-RO) according to the manufacturer’s protocol.

    Article Title: YAP/TAZ-VGLL3 governs adipocyte fate via epigenetic reprogramming of PPARγ and its target enhancers
    Article Snippet: .. For generation of recombinant viruses, HEK293T cells were transfected with the use of PEI MAX (24765-1, Polysciences) for 48 hours either with a transfer plasmid together with Gag/Pol (#14887, Addgene; RRID: Addgene_14887) and vesicular stomatitis virus glycoprotein (#14888, Addgene; RRID: Addgene_14888) plasmids for retrovirus production or with psPAX2 (#12260, Addgene; RRID: Addgene_12260) and pMD2.G (#12259, Addgene; RRID: Addgene_12259) for lentivirus production. .. The virus-containing culture supernatants were harvested for infection of C3H10T1/2 cells for 24 hours in the presence of polybrene (H9268, Sigma-Aldrich) at 6 μg/ml.

    Article Title: Matrix Rigidity Controls Endothelial Differentiation and Morphogenesis of Cardiac Precursors
    Article Snippet: The p190RhoGAP overexpression plasmid was prepared by cloning the human open reading frame(ORF)segment (OHS1770) (Open Biosystems) in a simian virus 40 (SV40) lentiviral expression vector. .. Lentiviral shRNA transduction Human embryonic kidney (HEK) cells were plated at 50% confluency and transfected with pLKO1-based shRNA expression plasmid, vesicular stomatitis virus glycoprotein (VSVG) (Addgene, 8454), and Δ8.2 dvpr (Addgene, 8455) with FuGENE 6 (Roche, 11814443001). ..

    Article Title: Development of a monoclonal antibody to study MARCH6, an E3 ligase that regulates proteins that control lipid homeostasis.
    Article Snippet: .. On day 1, cells were transfected with three plasmids: i) 5 μg puromycin-selectable lentiCRISPRv2 plasmid encoding a guide targeting either exon 15 or exon 4 of MARCH6 as described above; ii) 3 μg psPAX2 plasmid encoding HIV gag polymerase (Addgene, Cat# 12260); and iii) 2 μg pMD2.G plasmid encoding vesicular stomatitis virus glycoprotein (Addgene, Cat# 12259). .. Transfections were carried out using X-tremeGENE HP (Sigma, Cat# XTGHPRO) according to the manufacturer’s protocol.

    Article Title: Serine protease 35 regulates the fibroblast matrisome in response to hyperosmotic stress.
    Article Snippet: Plasmids with nontargeting (#VSC11657; Horizon Discovery, Waterbeach, UK) and PRSS35-targeting (#V3SH11252229744426 and #V3SH11252-230227744; Horizon Discovery) shRNAs were commercially obtained. .. Lentiviruses packaged with PAX2 (#12260; Addgene) and vesicular stomatitis virus glycoprotein (#8454; Addgene) were collected from human embryonic kidney 293T cell supernatants 2 days after transfection. .. Viral supernatant was filtered through a 0.45-μm filter, supplemented with polybrene (8 μg/ml; #sc-134220; Santa Cruz Biotechnology, Santa Cruz, CA) and used for overnight (O/N) treatment of HDFs.

    Recombinant:

    Article Title: YAP/TAZ-VGLL3 governs adipocyte fate via epigenetic reprogramming of PPARγ and its target enhancers
    Article Snippet: .. For generation of recombinant viruses, HEK293T cells were transfected with the use of PEI MAX (24765-1, Polysciences) for 48 hours either with a transfer plasmid together with Gag/Pol (#14887, Addgene; RRID: Addgene_14887) and vesicular stomatitis virus glycoprotein (#14888, Addgene; RRID: Addgene_14888) plasmids for retrovirus production or with psPAX2 (#12260, Addgene; RRID: Addgene_12260) and pMD2.G (#12259, Addgene; RRID: Addgene_12259) for lentivirus production. .. The virus-containing culture supernatants were harvested for infection of C3H10T1/2 cells for 24 hours in the presence of polybrene (H9268, Sigma-Aldrich) at 6 μg/ml.

    shRNA:

    Article Title: Matrix Rigidity Controls Endothelial Differentiation and Morphogenesis of Cardiac Precursors
    Article Snippet: The p190RhoGAP overexpression plasmid was prepared by cloning the human open reading frame(ORF)segment (OHS1770) (Open Biosystems) in a simian virus 40 (SV40) lentiviral expression vector. .. Lentiviral shRNA transduction Human embryonic kidney (HEK) cells were plated at 50% confluency and transfected with pLKO1-based shRNA expression plasmid, vesicular stomatitis virus glycoprotein (VSVG) (Addgene, 8454), and Δ8.2 dvpr (Addgene, 8455) with FuGENE 6 (Roche, 11814443001). ..

    Transduction:

    Article Title: Matrix Rigidity Controls Endothelial Differentiation and Morphogenesis of Cardiac Precursors
    Article Snippet: The p190RhoGAP overexpression plasmid was prepared by cloning the human open reading frame(ORF)segment (OHS1770) (Open Biosystems) in a simian virus 40 (SV40) lentiviral expression vector. .. Lentiviral shRNA transduction Human embryonic kidney (HEK) cells were plated at 50% confluency and transfected with pLKO1-based shRNA expression plasmid, vesicular stomatitis virus glycoprotein (VSVG) (Addgene, 8454), and Δ8.2 dvpr (Addgene, 8455) with FuGENE 6 (Roche, 11814443001). ..

    Expressing:

    Article Title: Matrix Rigidity Controls Endothelial Differentiation and Morphogenesis of Cardiac Precursors
    Article Snippet: The p190RhoGAP overexpression plasmid was prepared by cloning the human open reading frame(ORF)segment (OHS1770) (Open Biosystems) in a simian virus 40 (SV40) lentiviral expression vector. .. Lentiviral shRNA transduction Human embryonic kidney (HEK) cells were plated at 50% confluency and transfected with pLKO1-based shRNA expression plasmid, vesicular stomatitis virus glycoprotein (VSVG) (Addgene, 8454), and Δ8.2 dvpr (Addgene, 8455) with FuGENE 6 (Roche, 11814443001). ..

    other:

    Article Title: YAP/TAZ-VGLL3 governs adipocyte fate via epigenetic reprogramming of PPARγ and its target enhancers.
    Article Snippet: For the establishment of stable cell lines, the infected cells were exposed for 1 week to puromycin (A1113803, Gibco) at 2 μg/ ml, G418 (G0175.0005, Duchefa) at 400 μg/ml, or blasticidin (A1113903, Gibco) at 10 μg/ml.



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